| West of Scotland Cup 2009-10 | ||||
| First Round | ||||
| Ardeer Thistle | Ayrshire | v | Dalry Thistle | Ayrshire |
| Auchinleck Talbot | Ayrshire | v | Blantyre Victoria | Central |
| Beith Juniors | Ayrshire | v | Thorniewood United | Central |
| Bellshill Athletic | Central | v | Cumnock Juniors | Ayrshire |
| Carluke Rovers | Central | v | Pollok | Central |
| Clydebank | Central | v | Forth Wanderers | Central |
| Cumbernauld United | Central | v | Neilston | Central |
| Darvel Juniors | Ayrshire | v | Irvine Meadow X1 | Ayrshire |
| East Kilbride Thistle | Central | v | Kilwinning Rangers | Ayrshire |
| Girvan | Ayrshire | v | Cambuslang Rangers | Central |
| Johnstone Burgh | Central | v | Arthurlie | Central |
| Kelo Rovers | Ayrshire | v | Larkhall Thistle | Central |
| Kilbirnie Ladeside | Ayrshire | v | Royal Albert | Central |
| Kirkintilloch Rob Roy | Central | v | Vale of Leven | Central |
| Lanark United | Central | v | Annbank United | Ayrshire |
| Largs Thistle | Ayrshire | v | Dunipace Juniors | Central |
| Lesmahagow | Central | v | Kilsyth Rangers | Central |
| Lugar Boswell Thistle | Ayrshire | v | Benburb | Central |
| Maryhill | Central | v | Rutherglen Glencairn | Central |
| Maybole Juniors | Ayrshire | v | Yoker Athletic | Central |
| Newmains United | Central | v | Ashfield | Central |
| Petershill | Central | v | Hurlford United | Ayrshire |
| Port Glasgow Juniors | Central | v | Greenock | Central |
| Saltcoats Vics | Ayrshire | v | Glasgow Perthshire | Central |
| Shettleston | Central | v | Craigmark Burntonians | Ayrshire |
| St. Anthony's | Central | v | Shotts Bon Accord | Central |
| St. Roch's | Central | v | Whitletts Victoria | Ayrshire |
| Troon | Ayrshire | v | Renfrew | Central |
| Vale of Clyde | Central | v | Glenafton Athletic | Ayrshire |
| Wishaw | Central | v | Ardrossan Winton Rovers | Ayrshire |
| BYES : Irvine Victoria & Muirkirk Juniors | ||||
| The above two were originally drawn to be played against each other before they realised | ||||
| they would need to be given a bye to enable 32 to be in the second round. | ||||